blastema cells derived from rabbit ear show stem cell characteristics

Authors

zahra mahmoudi

maryam moghaddam matin

morvarid saeinasab

saeideh nakhaei-rad

abstract

abstract regeneration is a biological phenomenon, which takes place via two main mechanisms: first, dedifferentiation of mature cells followed by their differentiation into functional new cells and second, activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. one of the best examples of healing process in mammals is the regeneration of damaged pinna in rabbits by blastema tissue. the aim of present study was to investigate culture requirements, proliferative properties and expression of some stemness factors in cells derived from regenerating blastema tissue obtained from rabbit pinna in vitro. the regenerating tissues were obtained from male new zealand white rabbits by double punching of the pinna and cell culture conditions were set to derive and enrich the self renewing cells for further characterisation. the cells were subjected to survival and growth examinations in vitro, and expression of several stemness factors was studied in these cells using reverse transcription polymerase chain reaction (rt-pcr). results revealed that the derived cells are rather immortal, as they have been growing for more than 120 passages in culture up until this report. furthermore, rt-pcr and flow cytometry analyses showed that these cells express a number of stemness related genes including oct4 and sox2. in conclusion, in this study, stem like cells were derived from blastema tissue of rabbit ears for the first time, showing great self renewing capacity, which provides a suitable in vitro model for regeneration studies. moreover, they could be considered as a good source of stem like cells for future applications.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Blastema from rabbit ear contains progenitor cells comparable to marrow derived mesenchymal stem cells

Rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. The purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (MSCs). Five New Zealand white male rabbits were used in the present study. A 2-mm hole was created in the a...

full text

blastema from rabbit ear contains progenitor cells comparable to marrow derived mesenchymal stem cells

rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. the purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (mscs). five new zealand white male rabbits were used in the present study. a 2-mm hole was created in the a...

full text

Blastema from rabbit ear contains progenitor cells comparable to marrow derived mesenchymal stem cells

Rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. The purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (MSCs). Five New Zealand white male rabbits were used in the present study. A 2-mm hole was created in the a...

full text

Effects of rabbit pinna-derived blastema cells on tendon healing

Objective(s): Tendon healing is substantially slow and often associated with suboptimal repair. Cell therapy is one of the promising methods to improve tendon repair. Blastema, a population of undifferentiated cells, represents characteristics of pluripotent mesenchymal stem cells and has the potentials to be used in regenerative medicine. The aim of this study was to ...

full text

Isolation and Characterization of the Progenitor Cells From the Blastema Tissue Formed at Experimentally-Created Rabbit Ear Hole

  Objective(s): Throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. Blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. The objective of the present study is to isolate, culture expa...

full text

Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells

Objective(s): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. Materials and Methods: ...

full text

My Resources

Save resource for easier access later


Journal title:
journal of cell and molecular research

جلد ۳، شماره ۱، صفحات ۲۵-۰

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023